Outreach
CREAM mentors high school and undergraduate students in metabolomics research, with opportunities to develop projects and present findings.
Student research opportunities
CREAM is committed to inspiring the next generation of scientists through hands-on research experiences. High school and undergraduate students work alongside UofL faculty mentors on metabolomics projects, developing analytical methods and presenting their findings at regional science fairs and academic conferences.
Outreach programs
High school and junior high school students sponsored and mentored by University of Louisville faculty listed to work on the projects of the Metabolomics Initiative. All authored and presented posters at various regional events like the Regional Science Fair, Dupont Manual High School.
Past students
| Student | Title of Poster/Award | Date Presented | Mentor |
|---|---|---|---|
| Camille R. | 2012 | Hunter Moseley | |
| Alison D. | The Lengths of the Proteins in the Collagen Family compared to the Number of Single Nucleotide Polymorphisms | 2011 | Hunter Moseley |
| Rodney F. | "Developing Computational Tools for Molecular Comparison and Metabolic Placement of Detectable Uncharacterized Metabolites" James Graham Brown Cancer Center 10th Annual Retreat | 2010 | Hunter Moseley |
| A. D. | Urine microvesicles from cancer patients as biomarkers | 2010 | Andrew Lane |
| Monali H. | "The Effect of the Metabolic Inhibitors 2‐deoxyglucose and Dehydroepiandrosterone on Glucose Uptake and Lactate Secretion in A549 Human Lung Adenocarcinoma Cells" Regional and State Science Fair winner | 2008 | Andrew Lane |
| A. D. | "The Effect of the Metabolic Inhibitors 2‐deoxyglucose and Dehydroepiandrosterone on Glucose Uptake and Lactate Secretion in A549 Human Lung Adenocarcinoma Cells" Regional and State Science Fair winner | 2008 | Andrew Lane |
| Rosslyn S. | Effect of Potassium on the Level of Unsaturation of Soybean Lipids | 2007 | Cecilia Yappert |
| Nambi A. | The Effect of Cadmium on Lung Cancer Cells (A549) | 2007 | Teresa Fan |
| Li Z. | The Effect of Zinc on Rate of Fermentation | 2006 | Teresa Fan |
| Nambi A. | Effect of Selenium on Breast Cancer Cells (HTB29) | 2006 | Teresa Fan |
| Doug L. | Electrophoretic separation of proteins | 2005 | Teresa Fan |
| Patrick O. | Cancer Cell Response to Selenium | 2005 | Teresa Fan |
Many University of Louisville undergraduate students, and some from other institutions within Kentuckiana, receive hands-on instruction at CREAM. Undergraduate students participate in all aspects of research including conducting their own projects, developing analytical methods, and preparing platform and poster presentations.
Past students
| Student | Project | Dates | Mentor |
|---|---|---|---|
| Connor Smith | 2014 | Xiang Zhang | |
| Brent Hukill | Stable isotope labeling resolved sample complexing for high throughput metabolomics | 2013 | Xiang Zhang |
| Craig Clemons | 2010–2013 | Hunter Moseley | |
| Andrew McCollam | 2011–2013 | Hunter Moseley | |
| Eugene Hinderer | 2011–2013 | Hunter Moseley | |
| John Taylor Hans | 2011–2013 | Hunter Moseley | |
| Annabelle Carrell | 2011–2013 | Hunter Moseley | |
| Arren Carter | 2011–2013 | Hunter Moseley | |
| Indraneel Reddy | 2011–2013 | Hunter Moseley | |
| Patrick Mullaney | 2008–2012 | Hunter Moseley | |
| Abigail Hoskins | 2008–2012 | Hunter Moseley | |
| Sabrina Shatzman | 2009–2012 | Teresa Fan | |
| Joshua Mitchell | Developing Computational Tools for Molecular Comparison and Metabolic Placement of Detectable Uncharacterized Metabolites | 2010–2012 | Hunter Moseley |
| Elizabeth Lorch | 2011–2012 | Hunter Moseley | |
| Zelalem Mekonnen | 2011 | Teresa Fan | |
| Austin McCuiston | Lipidomics of Microvesicles in Human Lung Cancer Cells for Cancer Detection | 2011 | Andrew Lane |
| Joshua Mabrey | 2011 | Hunter Moseley | |
| Timothy Cook | 2008–2011 | Hunter Moseley | |
| Rima Patel | 2010–2011 | Hunter Moseley | |
| G. Bousamra | 31P NMR based energy metabolism in rat tissue | 2010 | Teresa Fan |
| Alex Belshoff | Arsenic Detoxification: Inhibition of Glutathione Production Results in Increased Arsenic Accumulation | 2007– 2008 | Teresa Fan |
| Kathleen Marshall | 2007–2008 | Teresa Fan | |
| Mark Capece | 2008 | Teresa Fan | |
| Ben King | 2008 | Teresa Fan | |
| M. Habib | Energy metabolism in rat skeletal muscle | 2008 | Andrew Lane |
| Martin McKinney | Stable Isotope Resolved Metabolomics Analysis of Ribonucleotide and RNA Metabolism | 2008 | Andrew Lane |
| Sabine Dhakal | 2007 | Teresa Fan | |
| Josh Campbell | Effect of Phosphorus on the Level of Unsaturation of Soybean Lipids | Cecilia Yappert | |
| Marchello Cavitt | Analysis of Soybean Lipids by TLC and in situ MALDI MS | Cecilia Yappert | |
| Nichole Sonderman | Effect of Thermal Oxidation of Soybean Triacylglycerides | Cecilia Yappert | |
| Maresha Williams | Cecilia Yappert |
Introducing metabolomics to a diverse community
CREAM faculty and staff have also participated in many local conferences with audiences ranging from secondary school teachers to medical doctors. These presentations serve to educate the community about the discipline of metabolomics and offer the instructional services of the center to the community it serves.
- October 2014 – Department of Oral Immunology and Infectious Diseases, Dental School, University of Louisville: Xiang Zhang presenting "Metabolomics Program at CREAM"
- January 2012 – Birth Defects Center, University of Louisville School of Medicine: Xiang Zhang presenting " Metabolomics Analysis Reveals Reverse Fatty Acid Transport in Alcohol Induced Fatty Liver"
- August 2011 – Summer Student Program, JG Brown Cancer Center: “Clinical Applications of Stable Isotope-Resolved Metabolomics (SIRM) in Non Small Cell Lung Cancer”
- March 2011 – IMD3 Symposium 2011, Xiang Zhang presenting "Metabolomics as a key to deciphering molecular regulation in liver injury"
- Fall 2010 – Berea College: Teresa Fan presenting "Metabolism: Old or Gold?"
- Fall 2010 – Huntingdon College: Hunter Moseley presenting "Metabolic Modeling of Converging Metabolic Pathways. Analysis of Non-Steady State Stable Isotope-Resolved Metabolomics of UDP-GlcNAc and UDP-GalNAc"
- Nov. 21, 2009 – JG Brown Cancer Center and Marky Cancer Center Colloquium, CTRB Louisville: Teresa Fan presenting "Translating Metabolomics into Novel Cancer Therapeutics"
- Sept. 15, 2007 – Kentucky Association for Environmental Education: Teresa Cassel presenting "Metabolomics in bioavailability, mechanism, and biomarker discovery"
- Nov. 2, 2007 – Kentucky Science Teachers Association: Teresa Cassel presenting "Metabolomics and Isotopomer analysis".
Visiting researchers
Developing research scientists of all levels are welcome to work side-by-side with CREAM core faculty and staff learning metabolomics techniques and applying them to their particular research interests. Students and junior faculty from laboratories across campus, around the state, throughout the nation and from around the world contribute to the interdisciplinary cross-pollination of ideas that is a very important CREAM objective.
- Sandra Gomes – Sao Paulo. Sample preparation for SIRM studies of glutaminase C in cancer cells.
- Scott Jones – Medicine at University of Louisville. Natural products involved in pre-Parkinson’s disease symptoms.
- Anne Le – Johns Hopkins University. SIRM analysis of MYC expression in cancer cells.
- Santigo Moralli – University of Barcelona. High-throughput metabolite analysis, specifically the study of the lipidome and its changes during cell cycle progression and tumor transformation.
- Jason Podrabsky – Oregon State. GC-MS analysis of killifish embryos.
- Miriam Porquet – University of Barcelona. Study of metabolic and lipid profiles of mouse embryogenic fibroblast cell lines using LTQ FT MS and GCMS techniques and software to describe the metabolic importance of the lack of two key enzymes (CDK4 and CDK6) in the cell cycle.
- Chris Ricketts and Youfeng Yang – NCI SIRM studies of metabolic reprogramming in fumarate hydrate null cells.
- Pankaj Seth – Beth Israel Deaconess, Boston. SIRM analysis of LDH-A knockdowns in cancer cells.
- Mariia Yuneva – University of California, San Francisco. Molecular regulatory mechanisms of Myc oncogene in mammalian cells.
Professional symposia
Bringing the most current and innovative work in the metabolomics discipline to students and scientists in the Louisville area is an important mission of the center. Three metabolomics symposia have been hosted by the center:
- Nov. 5-6, 2005 – First Louisville Symposium on Metabolomics: Program, sponsors
- March 24-25, 2007 – Second International Metabolomics Symposium: Program (with abstracts),sponsors
- March 8-9, 2009 – Mini Symposium on Informatics for Metabolomics, Louisville: Program