KSCIRC Neuroscience Cores
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Core A - Project Development and Analysis
Program Director
Charles Hubscher, Ph.D.
Executive Director
Brenda Lujan, M.B.A.
Personnel
- Administer and oversee the Core facilities. Includes scheduling and documenting core-related meetings, all core-related space and physical plant, and personnel issues.
- Grant and experimental design mentorship by senior KSCIRC faculty. Grants and experiments will be pre-reviewed by KSCIRC faculty.
- Engineering support. Provide engineering support with prototyping and design assistance.
Core B - Surgery & Animal Care
Personnel
Christine Armstrong
- provide standardized rat and mouse surgery and other animal procedures
- provide extensive training for research personnel on surgical procedures, anesthesia, animal pre- and post-op care and the use of the three different spinal cord injury devices present in the Core
- assist laboratories to develop and establish novel surgical procedures
Core C - Behavioral and Electrophysiological Assessment
Personnel
Johnny Morehouse
- provide a knowledge base and expertise to facilitate standardized behavioral and electrophysiological assessments of rats and mice.
- train COBRE/KSCIRC PIs to perform high quality behavioral and electrophysiological assessments
- assist with the development of novel behavioral and electrophysiological assessment techniques.
Core D - Cell and Tissue Imaging and Histology (CTIH)
Personnel
Kariena Andres
- 350 sq ft total of microscopy & histology facility that includes a Nikon C2+ confocal microscope with intelligent acquisition, deconvolution, 3D measurement package, artificial intelligence image processing, LUN4 solid state laser launch (405, 488, 561, and 640 nm), DUVb high-sensitivity GaAsP detectors, an additional ORCA-Fusion Gen-III sCMOS monochrome camera, anti-vibration table, and a high performance Windows10 workstation with 31.5” 4K ultraHD display.
- The core also includes a Nikon TiE inverted microscope coupled to a Andor NeoZyla sCMOS camera for fluorescence imaging, as well as a Nikon DS-U2 color camera for phase and bright field imaging, Nikon Elements Generation 3 software and Image-Pro Plus software (Media Cybernetics). A Zeiss Axio Observer.Z1 inverted microscope equipped with Apotome and a CO2 chamber with heated plate lids for live imaging and Ca2+ fluorescence.
- There is one Leica CM3050 cryostats for use